Cloned (Comment) | Organism |
---|---|
1,2RhaT cloning and DNA and amino acid sequence determination and analysis, phylogenetic analysis, two native frameshift mutations render the enzyme from orange catalytically inactive | Citrus sinensis |
phylogenetic analysis, 1,2RhaT expressionin BY-2 cells | Citrus maxima |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Citrus maxima | the enzyyme specifically catalyzes rhamnosylation of flavanone-7-O-glucose and flavone-7-O-glucose substrates at position 2 of the glucose moiety | ? | - |
? | |
UDP-L-rhamnose + diosmetin 7-O-glucoside | Citrus maxima | a flavone 7-O-glucoside | UDP + diosmetin 7-O-[beta-L-rhamnosyl-(1-2)-beta-D-glucoside] | i.e. neodiosmin, a bitter 7-O-neohesperidoside | ? | |
UDP-L-rhamnose + hesperetin 7-O-glucoside | Citrus maxima | - |
UDP + hesperetin 7-O-[beta-L-rhamnosyl-(1-2)-beta-D-glucoside] | i.e. neohesperidin, a bitter 7-O-neohesperidoside | ? | |
UDP-L-rhamnose + naringenin 7-O-glucoside | Citrus maxima | - |
UDP + naringenin 7-O-[beta-L-rhamnosyl-(1-2)-beta-D-glucoside] | i.e. naringin, a bitter 7-O-neohesperidoside | ? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Citrus maxima | - |
- |
- |
Citrus sinensis | - |
- |
- |
Citrus sinensis valencia | - |
- |
- |
no activity in Citrus reticulata | - |
- |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
fruit | - |
Citrus maxima | - |
leaf | - |
Citrus maxima | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | the enzyyme specifically catalyzes rhamnosylation of flavanone-7-O-glucose and flavone-7-O-glucose substrates at position 2 of the glucose moiety | Citrus maxima | ? | - |
? | |
additional information | the enzyme also catalyzes the rhamnosylation of diosmetin 7-O-glucoside at position 2 of the glucose moiety to give neodiosmin, but Cm1,2RhaT is specific for flavonoid-7-O-glucosides and utilizes no flavonoid-3-O-glucosides | Citrus maxima | ? | - |
? | |
UDP-L-rhamnose + diosmetin 7-O-glucoside | a flavone 7-O-glucoside | Citrus maxima | UDP + diosmetin 7-O-[beta-L-rhamnosyl-(1-2)-beta-D-glucoside] | i.e. neodiosmin, a bitter 7-O-neohesperidoside | ? | |
UDP-L-rhamnose + hesperetin 7-O-glucoside | - |
Citrus maxima | UDP + hesperetin 7-O-[beta-L-rhamnosyl-(1-2)-beta-D-glucoside] | i.e. neohesperidin, a bitter 7-O-neohesperidoside | ? | |
UDP-L-rhamnose + hesperetin 7-O-glucoside | a flavanone 7-O-glucoside | Citrus maxima | UDP + hesperetin 7-O-[beta-L-rhamnosyl-(1-2)-beta-D-glucoside] | i.e. neohesperidin, a bitter 7-O-neohesperidoside | ? | |
UDP-L-rhamnose + naringenin 7-O-glucoside | - |
Citrus maxima | UDP + naringenin 7-O-[beta-L-rhamnosyl-(1-2)-beta-D-glucoside] | i.e. naringin, a bitter 7-O-neohesperidoside | ? | |
UDP-L-rhamnose + naringenin 7-O-glucoside | a flavanone 7-O-glucoside | Citrus maxima | UDP + naringenin 7-O-[beta-L-rhamnosyl-(1-2)-beta-D-glucoside] | i.e. naringin, a bitter 7-O-neohesperidoside | ? |
Synonyms | Comment | Organism |
---|---|---|
1,2-rhamnosyltransferase | - |
Citrus sinensis |
1,2-rhamnosyltransferase | - |
Citrus maxima |
1,2RhaT | - |
Citrus sinensis |
1,2RhaT | - |
Citrus maxima |
flavanone-7-O-glucose-1,2-rhamnosyltransferase | - |
Citrus sinensis |
flavanone-7-O-glucose-1,2-rhamnosyltransferase | - |
Citrus maxima |
General Information | Comment | Organism |
---|---|---|
metabolism | the enzyme is involved in the biosynthesis of flavonoid branched-chain glycosides in citrus, overview. 1,2RhaT is the key branch-forming enzyme directing biosynthesis of the bitter flavanone neohesperidoside compounds in bitter Citrus species | Citrus maxima |